Colonels
- Colonel James Ellis Burton (Company H)
- Colonel John Coburn
Lieutenant Colonels
- Lieutenant Colonel James M. Henderson (Company F)
- Lieutenant Colonel John P. Niederauer (Company K)
Majors
- Major William Jenkins Manker
- Major John C. Maze (Companies D/G)
- Major Levin T. Miller (Company K)
Adjutants
- Adjutant James. H. Durham
- Adjutant John Randolph McBride (Company C)
- Adjutant Charles H. Pickering (Company E)
- Adjutant Charles H. Porter (Company I)
- Adjutant Estes Wallingford (Company E)
Quartermasters
- Quartermaster Heneage B. Finch
- Quartermaster Robert Milburn McMaster (Company F)
- Quartermaster John Adam Wilkens (Company E)
Chaplains
- Chaplain Joseph L. Irwin (Company A)
- Chaplain John McCrea
Medical Staff
- Surgeon Joseph Glass McPheeters
- Surgeon Robert F. Bence
- Assistant Surgeon John A. Fitzgerald
- Assistant Surgeon Andrew M. Hunt
- Assistant Surgeon John Moffitt
- Assistant Surgeon Jeremiah K. Vincent (Company C)
Captains
- Captain William A. Dilley (Company A)
- Captain Charles Seaton (Company A)
- Captain Andrew Thomas Wellman (Company A)
- Captain James Lewis Banks (Company B)
- Captain Benjamin H. Freeland (Company B)
- Captain John Thomas Freeland (Company B)
- Captain Charles Day (Company C)
- Captain John Hart (Company C)
- Captain Amos J. Thomas (Company C)
- Captain William Chandler (Company D)
- Captain Edward T. McCrea (Company D)
- Captain James Henry Brown (Company E)
- Captain Isaac C. Hendricks (Company E)
- Captain William A. Whitson (Company E)
- Captain Joseph Theodore Fleming (Company F)
- Captain William S. McCullough (Company F)
- Captain Burr H. Polk (Company F)
- Captain Israel C. Dille (Company G)
- Captain Samuel D. Helman (Company G)
- Captain John T. Slough (Company H)
- Captain Enos Halbert (Company I)
- Captain William Andrew Wilkinson Hauser (Company I)
- Captain George L. Scott (Company I)
- Captain Henry Clay Johnson (Company K)
Lieutenants
- 1st Lieutenant Henry R. Flook (Company A)
- 1st Lieutenant John W. Taylor (Company A)
- 2nd Lieutenant George W. Greenlee (Company A)
- 2nd Lieutenant James M. Simpson (Company A)
- 1st Lieutenant Israel M. Adams (Company B)
- 1st Lieutenant Andrew W. Fullerton (Company B)
- 1st Lieutenant William Wagoner Hollingsworth (Company B)
- 1st Lieutenant Henry H. Jeter (Company B)
- 2nd Lieutenant Eli Monroe Adams (Company B)
- 2nd Lieutenant Winfield S. Read (Company B)
- 1st Lieutenant William J. Day (Company C)
- 1st Lieutenant John Hardwick (Company C)
- 2nd Lieutenant James Gallagher Bain (Company C)
- 2nd Lieutenant Andrew J. Cox (Company C)
- 1st Lieutenant David A. Fateley (Company D)
- 2nd Lieutenant William Henry Harrison Miller (Company D)
- 2nd Lieutenant John E. Smith (Company D)
- 1st Lieutenant Andrew Jackson Buchanan (Company E)
- 1st Lieutenant James Nathaniel Hill (Company E)
- 1st Lieutenant John Robert Spratt (Company E)
- 2nd Lieutenant Lloyd T. Dunkin (Company E)
- 2nd Lieutenant Hezekiah Harrison Lyon (Company E)
- 1st Lieutenant James Cook McClurkin (Company F)
- 2nd Lieutenant Francis Brunson (Company F)
- 2nd Lieutenant Robert F. McConnell (Company F)
- 1st Lieutenant Isaac J. Betts (Company G)
- 1st Lieutenant William Farrell (Company G)
- 1st Lieutenant Plina McKnight (Company G)
- 2nd Lieutenant William H. Boone (Company G)
- 2nd Lieutenant Augustus J. Horton (Company G)
- 1st Lieutenant Henry Burkhart (Company H)
- 1st Lieutenant Lawson Emanuel McKinney (Company H)
- 2nd Lieutenant James Henry Collier (Company H)
- 2nd Lieutenant Jefferson Coleman Farr (Company H)
- 1st Lieutenant Edwin I. Bachman (Company I)
- 1st Lieutenant Henry Logan Trisler (Company I)
- 2nd Lieutenant Jesse L. Crisler (Company I)
- 2nd Lieutenant John Ambrose Miller (Company I)
- 1st Lieutenant William Nodurft (Company K)
- 2nd Lieutenant Thomas Graves (Company K)
- 2nd Lieutenant John Wesley Slauter (Company K)
Sergeant Majors
- Sergeant Major Joseph R. Shelton (Company A)
Quartermaster Sergeants
- Quartermaster Sergeant William Mortimer Bennett (Company H)
- Quartermaster Sergeant James F. Mahan (Company G)
Commissary Sergeants
Hospital Stewards
- Hospital Steward Mordecai Redd (Company I)
Principle Musicians
- Drum Major Jefferson Hezekiah Foxworthy (Company H)
- Principle Musician Alexander C. Hamilton (Company A)
- Principle Musician Charles G. Michael (Company I)
- Principle Musician Frederick J. Niemann (Company G)
- Principle Musician Hiram Van Buren Parker (Company K)
- Principle Musician Reuben R. Ransom (Company K)